<li id="quy4e"></li>
<button id="quy4e"></button>
  • <button id="quy4e"><bdo id="quy4e"></bdo></button>
    <rt id="quy4e"></rt>
    <tfoot id="quy4e"></tfoot>
  • 咨詢熱線

    15000266580

    當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-11268293T 人胚腎細(xì)胞

    293T 人胚腎細(xì)胞

    簡(jiǎn)要描述:CRL-11268 293T 人胚腎細(xì)胞
    ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

    • 產(chǎn)品型號(hào):CRL-11268
    • 廠商性質(zhì):生產(chǎn)廠家
    • 更新時(shí)間:2025-04-27
    • 訪  問(wèn)  量:1914

    產(chǎn)品分類

    Product Category

    詳細(xì)介紹

    CRL-11268 293T 人胚腎細(xì)胞

    ATCC® Number:  CRL-11268™

    Designations:  293T/17 [HEK 293T/17]

    Depositors:   Rockefeller Univ.

    Biosafety Level: 2 [Cells contain Adeno and SV-40 viral DNA sequences ]

    Shipped:  frozen

    Medium & Serum:  See Propagation

    Growth Properties: adherent

    Organism: Homo sapiens (human)

    Morphology: epithelial

    CRL-11268 293T 人胚腎細(xì)胞

    Source: Organ: kidney

    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


    Restrictions: The line is available with the following restriction: 1. The cell line was deposited at the ATCC by Rockefeller University and is provided for research purposes only. Neither the cell line nor the products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the cells, or their products, must first be negotiated with Cell Genesys, 500 Forbes Boulevard, South San Francisco, CA 94080 Attn: Robert H. Tidwell; Senior Vice President, Corporate Development.

    Antigen Expression: SV40 T antigen [45408]

    Age:  fetus

    Comments: The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line.ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line.ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554) amphotropic envelope-expression packaging cell line.

    Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

    Temperature: 37.0°C

    CRL-11268

    Subculturing:  Protocol:

    Remove and discard culture medium.

    Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

    Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

    Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

    Add appropriate aliquots of the cell suspension to new culture vessels.

    Incubate cultures at 37°C.

    Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

    Medium Renewal: Every 2 to 3 days

    Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

    Storage temperature: liquid nitrogen vapor phase

    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

    recommended serum:ATCC 30-2020

    derivative:ATCC CRL-11269

    References: 45408: Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361

    57446: Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999

    57447: Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001

    57448: Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960




















    產(chǎn)品咨詢

    留言框

    • 產(chǎn)品:

    • 您的單位:

    • 您的姓名:

    • 聯(lián)系電話:

    • 常用郵箱:

    • 省份:

    • 詳細(xì)地址:

    • 補(bǔ)充說(shuō)明:

    • 驗(yàn)證碼:

      請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
    聯(lián)系方式

    郵箱:xiangfbio@163.com

    地址:上海市虹口區(qū)四平路710號(hào)7層

    咨詢熱線

    400-821-8510

    (周一至周日9:00- 19:00)

    在線咨詢
    • 掃一掃 微信咨詢

    Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
    技術(shù)支持:化工儀器網(wǎng)    管理登陸
    主站蜘蛛池模板: 赞皇县| 大兴区| 曲靖市| 曲麻莱县| 苗栗县| 潼南县| 句容市| 咸丰县| 旬阳县| 石林| 庐江县| 团风县| 兰州市| 大关县| 泽库县| 夏河县| 康定县| 石门县| 平舆县| 罗源县| 客服| 察隅县| 西峡县| 四子王旗| 宁晋县| 丹江口市| 同江市| 永胜县| 新民市| 马尔康县| 建昌县| 百色市| 高阳县| 信丰县| 安顺市| 黄骅市| 双城市| 即墨市| 西平县| 成武县| 色达县|