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    當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-3006JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

    JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

    簡要描述:CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

    • 產(chǎn)品型號:CRL-3006
    • 廠商性質(zhì):生產(chǎn)廠家
    • 更新時(shí)間:2025-05-25
    • 訪  問  量:2242

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    CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

    ATCC® Number:CRL-3006™    Price:$417.00
    Designations:JeKo-1

    Depositors:Elias Campo

    Biosafety Level:1

    Shipped:frozen

    Medium & Serum:See Propagation

    Growth Properties:suspension

    Organism:Homo sapiens (human)

    Morphology:lymphoblast

    Characterization Data



    Source:Organ: peripheral blood
    Tissue: Mantle Cell Lymphoma


    Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

    Antigen Expression:CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

    DNA Profile (STR):Amelogenin:X

    CSF1PO:9,12

    D13S317:8,9

    D16S539:12

    D5S818:10,13

    D7S820:10,11

    THO1:7

    TPOX:8

    vWA: 14



    Age:78

    Gender:female

    Comments:The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

    Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
    Atmosphere: 5% CO2 in air recommended
    Temperature: 37.0°C


    Subculturing:Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

    Preservation:Freeze medium: 30% RPMI; 60% FBS; 10% DMSO
    Storage temperature: liquid nitrogen vapor phase


    References:92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
    16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
    16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

    CRL-3006

    ATCC® Number:CRL-3006™    Price:$417.00
    Designations:JeKo-1

    Depositors: Elias Campo

    Biosafety Level:1

    Shipped:frozen

    Medium & Serum:See Propagation

    Growth Properties:suspension

    Organism:Homo sapiens (human)

    Morphology:lymphoblast

    Characterization Data



    Source:Organ: peripheral blood

    Tissue: Mantle Cell Lymphoma



    Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



    Antigen Expression:CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

    DNA Profile (STR):Amelogenin:X

    CSF1PO:9,12

    D13S317:8,9

    D16S539:12

    D5S818:10,13

    D7S820:10,11

    THO1:7

    TPOX:8

    vWA: 14



    Age:78

    Gender:female

    Comments:The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

    Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

    Atmosphere: 5% CO2 in air recommended

    Temperature: 37.0°C



    Subculturing:Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

    Preservation:Freeze medium: 30% RPMI; 60% FBS; 10% DMSO

    Storage temperature: liquid nitrogen vapor phase



    References:92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
    16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
    16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063




















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