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    當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>IMR-90人胚肺成纖維細(xì)胞

    人胚肺成纖維細(xì)胞

    簡要描述:CCL-186 IMR-90 人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

    • 產(chǎn)品型號:IMR-90
    • 廠商性質(zhì):生產(chǎn)廠家
    • 更新時(shí)間:2025-06-26
    • 訪  問  量:3164

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    CCL-186 IMR-90人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

    CCL-186 IMR-90人胚肺成纖維細(xì)胞.

    ATCC® Number: CCL-186™

    Designations: IMR-90

    Depositors: WW Nichols

    Biosafety Level: 1

    Shipped: frozen

    Medium & Serum: See Propagation

    Growth Properties: adherent

    Organism: Homo sapiens (human)

    Morphology: fibroblast

    CCL-186 IMR-90人胚肺成纖維細(xì)胞.

    Source: Organ: lung

    Disease: normal

    Cell Type: fibroblast

    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


    Applications: transfection host (Nucleofection technology from Lonza)

    Virus Susceptibility: Human poliovirus 1

    Human poliovirus 2

    Varicella-Zoster

    Herpes simplex virus 1

    Herpes simplex virus 2

    Human poliovirus 3

    Vaccinia virus

    Human herpesvirus 5

    Vesicular stomatitis virus

    Reverse Transcript: negative

    DNA Profile (STR): Amelogenin: X

    CSF1PO: 11,13

    D13S317: 11,13

    D16S539: 10,13

    D5S818: 12,13

    D7S820: 9,12

    THO1: 8,9.3

    TPOX: 8,9

    vWA: 16,19

    Cytogenetic Analysis: normal human female; diploid; stable

    Isoenzymes: G6PD, B

    Age: 16 weeks gestation

    Gender: female

    Ethnicity: Caucasian

    Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

    The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

    The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

    Atmosphere: air, 95%; carbon dioxide (CO2), 5%

    Temperature: 37.0°C

    Subculturing: Protocol:

    Remove and discard culture medium.

    Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

    Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

    Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

    Add appropriate aliquots of the cell suspension to new culture vessels.

    Incubate cultures at 37°C.

    Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

    Medium Renewal: Every 3 to 4 days

    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

    Storage temperature: liquid nitrogen vapor phase

    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

    recommended serum:ATCC 30-2020

    References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

    32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

    33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

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