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    當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CCL-219L1210 小鼠白血病細(xì)胞

    L1210 小鼠白血病細(xì)胞

    簡(jiǎn)要描述:CCL-219 L1210 小鼠白血病細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

    • 產(chǎn)品型號(hào):CCL-219
    • 廠商性質(zhì):生產(chǎn)廠家
    • 更新時(shí)間:2025-06-28
    • 訪  問(wèn)  量:1669

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    CCL-219 L1210 小鼠白血病細(xì)胞

    ATCC® Number:  CCL-219™

    Designations:  L1210

    Depositors:   EM Jensen

    Biosafety Level: 1

    Shipp:  frozen

    Medium & Serum:  See Propagation

    Growth Predoperties: suspension

    Organism: Mus musculus (mouse)

    Morphology: lymphoblast

    CCL-219 L1210 小鼠白血病細(xì)胞

    Source: Disease: lymphocytic leukemia

    Strain: DBA subline 212

    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


    Tumorigenic: Yes

    Reverse Transcript: positive

    Cytogenetic Analysis: modal number = 40; range = 32 to 80.

    Mouse karyotype with a diploid stemline number of 40 chromosomes.

    Age:  8 months

    Gender:  female

    Comments: Tested and found negative for ectromelia virus (mousepox).

    The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513]

    Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

    Temperature: 37.0°C

    Subculturing:  Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml.

    Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days

    Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

    Storage temperature: liquid nitrogen vapor phase

    Doubling Time:  8 to 10 hrs

    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

    recommended serum:ATCC 30-2040

    References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949.

    26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966.

    26056: . . Cancer Chemother. Rep. 51: 451-453, 1967.

    32923: Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865

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