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    當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>TIB-67J774A.1 小鼠腹水單核細(xì)胞瘤

    J774A.1 小鼠腹水單核細(xì)胞瘤

    簡(jiǎn)要描述:TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

    • 產(chǎn)品型號(hào):TIB-67
    • 廠商性質(zhì):生產(chǎn)廠家
    • 更新時(shí)間:2025-04-27
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    TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

    TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤

    ATCC® Number:TIB-67™  Price:$256.00
    Designations:J774A.1

    Depositors:P Ralph

    Biosafety Level:1

    Shipped:frozen

    Medium & Serum:See Propagation

    Growth Properties:adherent

    Organism:Mus musculus (mouse)

    Morphology:macrophage
    J774A.1 小鼠腹水單核細(xì)胞瘤


    Source:Tissue: ascites
    Strain: BALB/cN
    Disease: reticulum cell sarcoma
    Cell Type: monocyte/macrophage macrophage;


    Cellular Products:interleukin 1 beta

    lysozyme [1080]



    Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    TIB-67

    Isolation:La Jolla California, United States
    Isolation date: 1968


    Applications:Biological response [92560]

    transfection host (Roche FuGENE® Transfection Reagents)



    Receptors:complement (C3), expressed [1135]

    Fc receptor, IgG, high affinity I (Fcgr1), expressed [13710]



    Age:adult

    Gender:female

    Comments:J774A.1 cells are active in antibody dependent phagocytosis [Pubmed: 1101071]. Their growth is inhibited by dextran sulfate, PPD and LPS [Pubmed: 318922]. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

    Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C


    Subculturing:Protocol: Subcultures are prepared by scraping.

    For a 75 cm2 flask, remove all but 10 ml of the culture medium. (adjust volume accordingly for different culture vessels) Dislodge cells from the flask substrate with a cell scraper, aspirate and dispense into new flasks.


    Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
    Medium Renewal: Replace or add medium two or three times weekly


    Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase


    Doubling Time:17 hours

    Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

    recommended serum:ATCC 30-2020

    purified RNA:ATCC TIB-67R



    References:1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

    1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

    1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

    13710: Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886

    22827: Ralph P, et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975. PubMed: 1089721

    22896: Ralph P, Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975. PubMed: 1101071

    32697: Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902

    32883: Schissel SL, et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996. PubMed: 8702487

    92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

















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