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    當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1708QT6 鵪鶉?yán)w維肉瘤細(xì)胞

    QT6 鵪鶉?yán)w維肉瘤細(xì)胞

    簡(jiǎn)要描述:CRL-1708 QT6 鵪鶉?yán)w維肉瘤細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

    • 產(chǎn)品型號(hào):CRL-1708
    • 廠商性質(zhì):生產(chǎn)廠家
    • 更新時(shí)間:2025-06-28
    • 訪  問(wèn)  量:2930

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    CRL-1708 QT6 鵪鶉?yán)w維肉瘤細(xì)胞的詳細(xì)介紹

    CRL-1708 QT6 鵪鶉?yán)w維肉瘤細(xì)胞

    ATCC® Number:CRL-1708™  Price:$438.00
    Designations:QT6

    Depositors:C Moscovici

    Biosafety Level:1

    Shipped:frozen

    Medium & Serum:See Propagation

    Growth Properties:adherent

    Organism:Coturnix coturnix japonica (quail, Japanese)

    Morphology:fibroblast


    Source:Disease: fibrosarcoma
    Cell Type: methylcholanthrene induced


    Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



    Applications:transfection host (Roche FuGENE® Transfection Reagents)

    Virus Susceptibility:Rous sarcoma virus

    Avian encephalomyelitis virus

    Rabies virus

    Marek's disease virus



    Tumorigenic:Yes

    Propagation:ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
    Temperature: 37.0°C


    Subculturing:Protocol: Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximay 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in atrypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.Note: When subcultures are prepared using pipetting or mechanical means thecells adhere lightly to the flask substrate and exhibit a tendency toward clumping.
    Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
    Medium Renewal: Twice per week


    Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase


    Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

    recommended serum:ATCC 30-2030



    References:22385: Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

    CRL-1708


















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