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    當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2095 CAL 27 人舌鱗癌細(xì)胞

    CRL-2095 CAL 27 人舌鱗癌細(xì)胞

    簡要描述:CRL-2095 CAL 27 人舌鱗癌細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|細(xì)胞,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件。

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    • 更新時間:2025-06-28
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    CRL-2095 CAL 27 人舌鱗癌細(xì)胞

    ATCC® Number:CRL-2095™Price:
    Designations:CAL 27

    Depositors:C Cardona

    Biosafety Level:1

    Shipped:frozen

    Medium & Serum:See Propagation

    Growth Properties:adherent

    Organism:Homo sapiens (human)

    Morphology:epithelial


    Source:Organ: tongue
    Disease: squamous cell carcinoma


    Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



    Isolation:Isolation date: 1982

    Tumorigenic:Yes

    DNA Profile (STR):

    Amelogenin: X

    CSF1PO: 10,12

    D13S317: 10,11

    D16S539: 11,12

    D5S818: 11,12

    D7S820: 10

    THO1: 6,9.3

    TPOX: 8

    vWA: 14,17

    CRL-2095 CAL 27 人舌鱗癌細(xì)胞



    Cytogenetic Analysis:aneuploid; modal number = 43

    Age:56 years

    Gender:male

    Ethnicity:Caucasian

    Comments:

    CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).

    A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.



    Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C


    Subculturin


    Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor temperature


    Doubling Time:35 hrs

    Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

    recommended serum:ATCC 30-2020



    References:

    23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269
























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